ANGPTL3 BioAssay(TM) ELISA Kit (Angiopoietin-like Protein 3, Angiopoietin-5, ANG-5) (Human)

Product Overview

• Article Name: ANGPTL3 BioAssay(TM) ELISA Kit (Angiopoietin-like Protein 3, Angiopoietin-5, ANG-5) (Human)
• Biozol Catalog Number: USB-044515
• Supplier Catalog Number: 044515
• Alternative Catalog Number: USB-044515-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Seven proteins containing an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both characteristics of angiopoietins, have been identified and therefore designated angiopoietin-like proteins 1-7 (ANGPTL 1-7). ANGPTL3 and ANGPTL6 (Angiopoietin-related growth factor, AGF), among others, function to regulate angiogenesis and also appear to directly regulate lipid, glucose, and energy metabolism. ANGPTL3 plasma levels are increased in a mouse model of diabetes mellitus. Sample Type: Cell culture supernatant, plasma, serum Sensitivity: 75pg/ml Range 0.156-10ng/ml Specificity: Recognizes human ANGPTL3. Does not cross-react with mouse ANGPTL3, human ANGPTL4, human ANGPTL6, human ANGPTL7 or human ANGPTL2. Test Principle: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human ANGPTL3 in biological fluids. A monoclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a purified polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3,5,5-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples. Kit Components: Plate coated with human ANGPTL3 antibody, 1x96wells Wash Buffer 10X, 1x50ml Diluent 5X, 1x50ml Detection Antibody, 1x12ml Detector 100X (HRP Conjugated anti-rabbit IgG), 1x150ul Human ANGPTL3 Standard, lyophilized, 1x20ng Human ANGPTL3 QC sample, lyophilized, 1x vial TMB Substrate Solution, 1x12ml Stop Solution, 1x12ml Storage and Stability: Store at 4C. After standard reconstitution, prepare aliquots and store at -20C. Plate and reagents should reach room temperature before use.