Glutathione peroxidase 3 (GPX3, GSHPX3) is a selenium-dependent extracellular enzyme, protecting cells and enzymes from oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxides. GPX3 is involved in inflammatory bowel disease, in asthma and in diabetes. The activity of GPX3 is significantly reduced in the plasma and tissue of cancer patients. Recently, GPX3 was suggested to serve as a molecular marker for the diagnosis of clear cell type ovarian adenocarcinoma. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (direct and indirect): 1:2000-1:5000 Western Blot: 1:2000-1:5000 using ECL, suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk, suggested incubation time is 1 hour at room temperature Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
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