Progranulin BioAssay(TM) ELISA Kit (Rat) (Proepithelin, PEPI, PC Cell-derived Growth Factor)

Product Overview

• Article Name: Progranulin BioAssay(TM) ELISA Kit (Rat) (Proepithelin, PEPI, PC Cell-derived Growth Factor)
• Biozol Catalog Number: USB-045173
• Supplier Catalog Number: 045173
• Alternative Catalog Number: USB-045173-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The granulins are a family of cysteine-rich polypeptides, some of which have growth modulatory activity. The widespread occurrence of granulin mRNA in cells from the hematopoietic system and in epithelia implies important functions in these tissues. All 4 known human granulin-like peptides are encoded in a single precursor, progranulin, which has a highly conserved 12-cysteine backbone defining a consensus sequence that is repeated 7 times. Progranulin is a 593-amino acid glycoprotein, the mRNA of which is expressed in many epithelial cells both in vitro and in vivo. He and Bateman demonstrated that overexpression of the progranulin gene in SW-13 adrenal carcinoma cells and MDCK nontransformed renal epithelia resulted in transfection-specific secretion of progranulin, acquired clonogenicity in semisolid agar, and increased mitosis in monolayer culture, whereas diminution of progranulin gene expression impaired growth of these cells. They proposed that the rate of growth of some epithelia is proportional to the level of intrinsic progranulin gene expression, and that elevated progranulin gene expression confers a transformed phenotype on epithelial cells including anchorage independence in vitro and growth as tumors in nude mice. They also found that in murine transcutaneous puncture wounds, progranulin mRNA is expressed in the inflammatory infiltrate and is highly induced in dermal fibroblasts and endothelia following injury. When applied to a cutaneous wound, progranulin increased the accumulation of neutrophils, macrophages, blood vessels, and fibroblasts in the wound. It acted directly on isolated dermal fibroblasts and endothelial cells to promote division, migration, and the formation of capillary-like tubule structures, concluding that progranulin is, therefore, probably a wound-related growth factor. Due to its tumor-promoting activity and multiple functions on hematopoietic cells and endothelial cells, progranulin can be a novel marker for oncology and hematology. The Progranulin BioAssay(TM) ELISA Kit (Proepithelin, PEPI, PC Cell-derived Growth Factor) is to be used for the in vitro quantitative determination of rat progranulin in serum and cell culture supernatant. Assay Principle: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of rat progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3,5,5-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of progranulin in the samples. Kit Components: 045173A: Microtiter Strips, 1x96wells 045173B: Wash Buffer 10X, 2x30ml 045173C: ELISA Buffer 10X, 2x30ml 045173D: Detection Antibody, 1x20ul 045173E: IgG (HRP-Streptavidin), 1x12ug 045173F: Standard, 1x8ng 045173G: TMB Solution, 1x12ml 045173H: Stop Solution 1N Sulfuric Acid, 1x12ml Storage and Stability: Store *045173F powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.