RGS1, Recombinant, Human, aa1-209, His-Tag (B Cell Activation Protein BL34, Early Response Protein 1R20, Regulator of G-protein Signaling 1, 1R20, BL34, IER1)
Biozol Catalog Number:
USB-045457
Supplier Catalog Number:
045457
Alternative Catalog Number:
USB-045457-100
Manufacturer:
US Biological
Category:
Molekularbiologie
RGS1 is a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120aa motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Source: Recombinant corresponding to aa1-209 from human RGS1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~26kD (233aa) confirmed by MALDI-TOF AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMRAAAI STPKLDKMPG MFFSANPKEL KGTTHSLLDD KMQKRRPKTF GMDMKAYLRS MIPHLESGMK SSKSKDVLSA AEVMQWSQSL EKLLANQTGQ NVFGSFLKSE FSEENIEFWL ACEDYKKTES DLLPCKAEEI YKAFVHSDAA KQINIDFRTR ESTAKKIKAP TPTCFDEAQK VIYTLMEKDS YPRFLKSDIY LNLLNDLQAN SLK Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
26
Purity:
~90% (SDS-PAGE)
Form:
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.15M sodium chloride, 10% glycerol.
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