RPL8, Recombinant, Human, aa1-257, His-Tag (60S Ribosomal Protein L8)
Biozol Catalog Number:
USB-045459
Supplier Catalog Number:
045459
Alternative Catalog Number:
USB-045459-50
Manufacturer:
US Biological
Category:
Molekularbiologie
RPL8 (60S ribosomal protein L8) belongs to the ribosomal protein L2P family. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm and exists as a component of the 60S subunit where it is thought to play a role in aminoacyl-tRNA binding, specifically at the ribosomal subunit interface. Recombinant protein corresponding to aa1-257 from human RPL8, fused to His-tag at N-terminus, expressed in E. coli. Molecular Weight: ~30.2kD (277aa) confirmed by MALDI-TOF Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK TVQEKEN Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
30.2
Purity:
90% (SDS-PAGE)
Form:
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 300mM sodium chloride, 2mM EDTA, 50% glycerol.
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