Adenovirus Hexon Antigen Calibration Kit for 045730

Product Overview

• Article Name: Adenovirus Hexon Antigen Calibration Kit for 045730
• Biozol Catalog Number: USB-045731
• Supplier Catalog Number: 045731
• Alternative Catalog Number: USB-045731-1
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Adenovirus Hexon Antigen Capture BioAssay(TM) ELISA kit (045730) is a highly sensitive and specific semi-quantitative assay for the detection of adenovirus hexon antigen in a wide variety of sample matrices. To enhance the analytical utility of the ELISA kit, the Adenovirus Hexon Antigen Calibration Kit permits the generation of a hexon antigen calibration curve and estimation of hexon antigen concentration in a sample. Kit Components: 1. Adenovirus Hexon Antigen (Ad5) Concentrate, 1x0.4ml (125ng/ml), Store at 2-8 C 2. Adenovirus Hexon Antigen Diluent, 1x50ml, (1X) Procedure: The Adenovirus Hexon Antigen Concentrate is supplied at a concentration of 125ng/ml and requires dilution prior to use. For the generation of a calibration curve, prepare an initial dilution of 1:25 in the diluent supplied. This will result in an Ad5 hexon concentration of 5ng/ml. Serial 2-fold dilutions should then be prepared from the initial dilution. Depending upon the nature of the sample being tested, it may be necessary to prepare calibrator dilutions in the diluent/buffer used for the experiment (tissue culture medium, for example). To determine the amount of hexon in a sample, a standard curve is constructed as follows: Calculate the average value for each antigen concentration and then subtract the 0 ng/ml value from each. Plot the resulting data and read the unknown values by extrapolation. Alternatively, enter the data into a calculator or a software program and calculate the sample concentrations by linear regression. Storage and Stability: Store at 2-8C. Stable for 6 months after receipt.