IL-5, Recombinant, Mouse, His-Tag, aa21-133

Product Overview

• Article Name: IL-5, Recombinant, Mouse, His-Tag, aa21-133
• Biozol Catalog Number: USB-045976
• Supplier Catalog Number: 045976
• Alternative Catalog Number: USB-045976-20,USB-045976-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

IL5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. This interleukin produced by type-2 T helper cells and mast cells. It related hematopoietic cytokines that are important for allergic inflammation.. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release. Recombinant mouse IL-5, fused to His-Tag at C -terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa21-133 from mouse IL-5, fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~13.9kD (119aa) Amino Acid Sequence: MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG Biological Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range 1 ng/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 13.9
• NCBI: 034688
• UniProt: P04401
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.