SIRP gamma, Recombinant, Human, His-Tag, aa29-360

Product Overview

• Article Name: SIRP gamma, Recombinant, Human, His-Tag, aa29-360
• Biozol Catalog Number: USB-045977
• Supplier Catalog Number: 045977
• Alternative Catalog Number: USB-045977-10,USB-045977-20,USB-045977-50,USB-045977-100,USB-045977-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

SIRPG, also known as signal-regulatory protein gamma isoform 1, is a member of a closely related family of three cell surface receptors implicated in modulating immune/inflammatory responses. It is the only SIRP known to be expressed on T cells, CD56 bright NK cells and activated NK cells. Expression of SIRPG on T cells suggests a role as an accessory protein interacting with CD47-expressing antigen presenting cells. Engagement of CD47 by SIRPG was shown to induce apoptosis on T-cell and monocyte cell lines. Recombinant human SIRPG, fused to hIgG-His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa29-360 from human SIRP gamma, fused to hlgG-His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~64kD (574aa) Amino Acid Sequence: EEELQMI QPEKLLLVTV GKTATLHCTV TSLLPVGPVL WFRGVGPGRE LIYNQKEGHF PRVTTVSDLT KRNNMDFSIR ISSITPADVG TYYCVKFRKG SPENVEFKSG PGTEMALGAK PSAPVVLGPA ARTTPEHTVS FTCESHGFSP RDITLKWFKN GNELSDFQTN VDPTGQSVAY SIRSTARVVL DPWDVRSQVI CEVAHVTLQG DPLRGTANLS EAIRVPPTLE VTQQPMRVGN QVNVTCQVRK FYPQSLQLTW SENGNVCQRE TASTLTENKD GTYNWTSWFL VNISDQRDDV VLTCQVKHDG QLAVSKRLAL EVTVHQKDQS SDATP Biological Activity: When measured by its binding ability in a functional ELISA with human CD47, the ED50 range is 1ug/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 64
• NCBI: 061026
• UniProt: Q9P1W8
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.