IFN-alpha 1, Recombinant, Porcine, His-Tag, aa24-189

Product Overview

• Article Name: IFN-alpha 1, Recombinant, Porcine, His-Tag, aa24-189
• Biozol Catalog Number: USB-045978
• Supplier Catalog Number: 045978
• Alternative Catalog Number: USB-045978-20,USB-045978-50,USB-045978-100,USB-045978-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

IFN-alpha 1, also known as Interferon alpha-1, belongs to a family of cytokines with potent antiviral antiproliferative and immunomodulatory properties. IFNs were originally discovered as molecules that could reduce the ability of a normal virus to infect cells, a process called viral interference. IFNs have been classified into two major types of IFNs, type I and type II, based on their interactions to a specific cell surface receptor. The type I IFNs bind to the interferon alpha receptor, which consists of two subunits, IFNAR1 and IFNAR2. The IFN-alpha proteins are produced mainly by plasmacytoid dendritic cells (pDCs). They are mainly involved in innate immunity against viral infection. It is also made synthetically as medication in hairy cell leukemia. Recombinant porcine IFN-alpha 1, fused to His-Tag at C-terminal, was expressed in HEK293 and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa24-189 from porcine IFN-alpha 1, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~20.2kD (176aa) Amino Acid Sequence: CDLPQT HSLAHTRALR LLAQMRRISP FSCLDHRRDF GSPHEAFGGN QVQKAQAMAL VHEMLQQTFQ LFSTEGSAAA WNESLLHQFC TGLDQQLRDL EACVMQEAGL EGTPLLEEDS ILAVRKYFHR LTLYLQEKSY SPCAWEIVRA EVMRSFSSSR NLQDRLRKKE Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 20.2
• NCBI: 999558
• UniProt: Q6VAB8
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.