H7N9/HA (hemaggulutinin1) belongs to the influenza viruses hemagglutinin family. Influenza A virus subtype H7N9 (A/H7N9) is a bird flu strain of the species Influenza virus A Influenza and Influenza hemagglutinin (HA) or haemagglutinin is a type of hemagglutinin found on the surface of the influenza viruses. An H7N9 virus was first reported to have infected humans in March 2013, in China. The CDC estimates that the H7N9 virus has the greatest potential compared with other influenza A viruses to cause a pandemic, although the risk is low, because like other type A viruses, it is not easily transmitted between people in its current form. H7N9/HA is an antigenic glycoprotein and is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane. Recombinant Influenza A virus (A/Anhui/1-BALF_RG45/2013(H7N9)) HA1 protein, fused to His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa19-339 from Influenza A H7N9 Hemagglutinin, fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~36kD (330aa) Amino Acid Sequence: DKICLGH HAVSNGTKVN TLTERGVEVV NATETVERTN IPRICSKGKR TVDLGQCGLL GTITGPPQCD QFLEFSADLI IERREGSDVC YPGKFVNEEA LRQILRESGG IDKEAMGFTY SGIRTNGATS ACRRSGSSFY AEMKWLLSNT DNAAFPQMTK SYKNTRKSPA LIVWGIHHSV STAEQTKLYG SGNKLVTVGS SNYQQSFVPS PGARPQVNGL SGRIDFHWLM LNPNDTVTFS FNGAFIAPDR ASFLRGKSMG IQSGVQVDAN CEGDCYHSGG TIISNLPFQN IDSRAVGKCP RYVKQRSLLL ATGMKNVPEI PKGR Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
