Thrombospondin 4, Recombinant, Mouse, His-Tag, aa27-963

Article Name:	Thrombospondin 4, Recombinant, Mouse, His-Tag, aa27-963
Biozol Catalog Number:	USB-045982
Supplier Catalog Number:	045982
Alternative Catalog Number:	USB-045982-20,USB-045982-100
Manufacturer:	US Biological
Category:	Molekularbiologie

Thrombospondin-4, also known as THBS4, is a member of the thrombospondin protein family. This family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentamer and can bind to heparin and calcium. Among them Thrombospondin-4 binds a variety of matrix proteins including Collagens I, II, III, V, Laminin-1, Fibronectin, and Matrilin-2. And it plays a role in cardiovascularphysiology and neuronal development. It is up-regulated in the spinal cord following peripheral nerve injury where it contributes to presynaptic hypersensitivity and hyperalgesia. It is also up-regulated in muscle following denervation. It contributes to the development of inflammation and atherosclerosis by promoting macrophage and neutrophil adhesion to the vasculature. Recombinant mouse Thrombospondin-4, fused to His-Tag at C -terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa27-963 from mouse Thrombospondin 4, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~104.3kD (943aa) Amino Acid Sequence: QATPQVFDLL PSSSQRLNPS ALQPVLTDPT LHEVYLISTF KLQSKSSATI FGLYSSSDNS KYFEFTVMGR LNKAILRYLK NDGKIHLVVF NNLQLADGRR HRVLLRLSNL QRGDGSVELY LDCAQADSVR NLPRAFSGLT QNPESIELRT FQRKPQDFLE ELKLVVRGSL FQVASLQDCF LQQSEPLAAT STGDFNRQFL GQMTQLNQLL GEVKDLLRQQ VKETSFLRNT IAECQACGPL SFQSPTPNTL VPIAPPAPPT RPTRHCDSSP CFRGVRCTDT RDGFQCGPCP DGYTGNGITC SDVDECKYHP CYPGVRCVNL APGFRCDACP VGFTGPMVQG VGINFAKTNK QVCTDVDECQ NGACVLNSIC INTLGSYRCG PCKPGYTGDQ TRGCKTERSC RNPEQNPCSV HAQCIEERQG DVTCVCGVGW AGDGYVCGKD VDIDSYPDEE LPCSARNCKK DNCKYVPNSG QEDADRDGIG DACDEDADGD GILNEQDNCV LTHNIDQRNS DKDIFGDACD NCRMVLNNDQ KDTDGDGRGD ACDDDMDGDG IKNILDNCPR VPNRDQQDRD GDDVGDACDS CPDVSNPNQS DVDNDLVGDS CDTNQDSDGD GHQDSTDNCP TVINSSQLDT DKDGIGDECD DDDDNDGIPD LVPPGPDNCR LVPNPAQEDS NNDGVGDICE ADFDQDQVID HIDVCPENAE ITLTDFRAYQ TVVLDPEGDA QIDPNWVVLN QGMEIVQTMN SDPGLAVGYT AFNGVDFEGT FHVNTQTDDD YAGFIFGYQD SSSFYVVMWK QTEQTYWQAT PFRAVAEPGI QLKAVKSKTG PGEHLRNSLW HTGDTSDQVR LLWKDSRNVG WKDKVSYRWF LQHRPQVGYI RVRFYEGSEL VADSGVTIDT TMRGGRLGVF CFSQENIIWS NLKYRCNDTI PEDFQEFQTQ SFDRLDN Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:	104.3
NCBI:	035712
UniProt:	Q9Z1T2
Purity:	85% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
Form:	Supplied as a liquid in PBS, pH 7.4, 0.1mM PMSF, 10% glycerol.