EphB1, Recombinant, Human, His-Tag, aa18-540

Product Overview

• Article Name: EphB1, Recombinant, Human, His-Tag, aa18-540
• Biozol Catalog Number: USB-045985
• Supplier Catalog Number: 045985
• Alternative Catalog Number: USB-045985-20,USB-045985-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

EphB1 also known as Ephrin type-B receptor 1, is a member of the ephrin receptor subfamily of the protein tyrosine kinase family which 16 known receptors. EphB1 has been shown to bind ephrin-B2, ephrin-B1, ephrin-A3, ephrin-A1, ephrin-A4, and ephrin-B3. It binds to tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. The ephrin/Eph families also appear to play a role in angiogenesis. Recombinant human EPHB1, fused to His-Tag at C- terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa18-540 from human EphB1, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~59.2kD (529aa) Amino Acid Sequence: MEETLMDTRT ATAELGWTAN PASGWEEVSG YDENLNTIRT YQVCNVFEPN QNNWLLTTFI NRRGAHRIYT EMRFTVRDCS SLPNVPGSCK ETFNLYYYET DSVIATKKSA FWSEAPYLKV DTIAADESFS QVDFGGRLMK VNTEVRSFGP LTRNGFYLAF QDYGACMSLL SVRVFFKKCP SIVQNFAVFP ETMTGAESTS LVIARGTCIP NAEEVDVPIK LYCNGDGEWM VPIGRCTCKP GYEPENSVAC KACPAGTFKA SQEAEGCSHC PSNSRSPAEA SPICTCRTGY YRADFDPPEV ACTSVPSGPR NVISIVNETS IILEWHPPRE TGGRDDVTYN IICKKCRADR RSCSRCDDNV EFVPRQLGLT ECRVSISSLW AHTPYTFDIQ AINGVSSKSP FPPQHVSVNI TTNQAAPSTV PIMHQVSATM RSITLSWPQP EQPNGIILDY EIRYYEKEHN EFNSSMARSQ TNTARIDGLR PGMVYVVQVR ARTVAGYGKF SGKMCFQTLT DDDYKSELRE QLP Biological Activity: Measured by its binding ability in a functional ELISA with Human EFNB1. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 59.2
• NCBI: 004432
• UniProt: P54762
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.