ErbB4, Recombinant, Human, His-Tag, aa26-649

Article Name:	ErbB4, Recombinant, Human, His-Tag, aa26-649
Biozol Catalog Number:	USB-045991
Supplier Catalog Number:	045991
Alternative Catalog Number:	USB-045991-20,USB-045991-100
Manufacturer:	US Biological
Category:	Molekularbiologie

ErbB4, also known as Her4, is a receptor tyrosine kinase that is a member of the epidermal growth factor receptor family. ErbB family members serve as receptors for the EGF family of growth factors. It is a single-pass type I transmembrane protein with multiple furin-like cysteine rich domains, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. It is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ErbB4 ligands include the neuregulins, beta cellulin and heparin-binding EGF-like growth factor (HB-EGF). Monomeric ErbB4 binds its ligands with low affinity. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer. Single nucleotide polymorphisms in ERBB4 have also been found in a study of patients with familial amyotrophic lateral sclerosis type 19. Recombinant human ErbB4, fused to hIgG-His-Tag at C-terminal, was expressed in HEK293 and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa26-649 from human ErbB4, fused to hIgG-His-Tag at C-terminal expressed in HEK293. Molecular Weight: ~96.6kD (863aa) Amino Acid Sequence: QSVCAGTENK LSSLSDLEQQ YRALRKYYEN CEVVMGNLEI TSIEHNRDLS FLRSVREVTG YVLVALNQFR YLPLENLRII RGTKLYEDRY ALAIFLNYRK DGNFGLQELG LKNLTEILNG GVYVDQNKFL CYADTIHWQD IVRNPWPSNL TLVSTNGSSG CGRCHKSCTG RCWGPTENHC QTLTRTVCAE QCDGRCYGPY VSDCCHRECA GGCSGPKDTD CFACMNFNDS GACVTQCPQT FVYNPTTFQL EHNFNAKYTY GAFCVKKCPH NFVVDSSSCV RACPSSKMEV EENGIKMCKP CTDICPKACD GIGTGSLMSA QTVDSSNIDK FINCTKINGN LIFLVTGIHG DPYNAIEAID PEKLNVFRTV REITGFLNIQ SWPPNMTDFS VFSNLVTIGG RVLYSGLSLL ILKQQGITSL QFQSLKEISA GNIYITDNSN LCYYHTINWT TLFSTINQRI VIRDNRKAEN CTAEGMVCNH LCSSDGCWGP GPDQCLSCRR FSRGRICIES CNLYDGEFRE FENGSICVEC DPQCEKMEDG LLTCHGPGPD NCTKCSHFKD GPNCVEKCPD GLQGANSFIF KYADPDRECH PCHPNCTQGC NGPTSHDCIY YPWTGHSTLP QHAR Biological Activity: Measured by its binding ability in a functional ELISA with Human NRG1/HRG1. The ED50 range 0.2ug/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:	96.6
NCBI:	005226
UniProt:	Q15303
Purity:	90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
Form:	Supplied as a liquid in PBS, pH 7.4, 10% glycerol.