TNFRSF4, Recombinant, Human, His-Tag, aa29-214

Product Overview

• Article Name: TNFRSF4, Recombinant, Human, His-Tag, aa29-214
• Biozol Catalog Number: USB-045992
• Supplier Catalog Number: 045992
• Alternative Catalog Number: USB-045992-20,USB-045992-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

TNFRSF4, also known as tumor necrosis factor receptor superfamily member 4, is a T cell co-stimulatory molecule in the TNF receptor superfamily. This protein coordinates with other co-stimulatory substances (CD28, CD40, CD30, CD27 and 4-1BB) to control the activation of the immune response. It plays an important role in antigen-specific T cell expansion and survival. It is up-regulated on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7. This protein also regulates cytokine production from T cells, antigen presenting cells, natural killer cells and natural killer cells and regulate cytokine receptor signaling. Recombinant human TNFRSF4, fused to hIgG-His-Tag at C -terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa29-214 from human TNFRSF4, fused to hlgG-His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~46.9kD (425aa) Amino Acid Sequence: LHCVGDTYPS NDRCCHECRP GNGMVSRCSR SQNTVCRPCG PGFYNDVVSS KPCKPCTWCN LRSGSERKQL CTATQDTVCR CRAGTQPLDS YKPGVDCAPC PPGHFSPGDN QACKPWTNCT LAGKHTLQPA SNSSDAICED RDPPATQPQE TQGPPARPIT VQPTEAWPRT SQGPSTRPVE VPGGRA Biological Activity: Measured by its binding ability in a functional ELISA with mouse OX40 Ligand/TNFSF4. The ED50 range 0.15ug/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 46.9
• NCBI: 003318
• UniProt: P43489
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.