ALCAM, Recombinant, Mouse, His-Tag, aa28-527

Product Overview

• Article Name: ALCAM, Recombinant, Mouse, His-Tag, aa28-527
• Biozol Catalog Number: USB-046002
• Supplier Catalog Number: 046002
• Alternative Catalog Number: USB-046002-20,USB-046002-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

ALCAM (Activated leukocyte cell adhesion molecule), also known as CD166 antigen, is a member of the Ig CAM family within the immunoglobulin superfamily. It interacts with CD6 that promotes T-cell activation and proliferation and plays a role in the binding of T- and B-cells to activated leukocytes. While expressed in a wide variety of tissues, ALCAM is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. Recently, CD166 is regarded as a potential novel breast cancer indicator and therapeutic target. Recombinant mouse ALCAM, fused to hIgG-His-Tag at C -terminal, was expressed in Insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa28-527 from mouse ALCAM, fused to hlgG-His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~83.1kD (739aa) Amino Acid Sequence: WYTVNSAYGD TIVMPCRLDV PQNLMFGKWK YEKPDGSPVF IAFRSSTKKS VQYDDVPEYK DRLSLSENYT LSIANAKISD EKRFVCMLVT EDNVFEAPTL VKVFKQPSKP EIVNKAPFLE TDQLKKLGDC ISRDSYPDGN ITWYRNGKVL QPVEGEVAIL FKKEIDPGTQ LYTVTSSLEY KTTRSDIQMP FTCSVTYYGP SGQKTIYSEQ EIFDIYYPTE QVTIQVLPPK NAIKEGDNIT LQCLGNGNPP PEEFMFYLPG QPEGIRSSNT YTLTDVRRNA TGDYKCSLID KRNMAASTTI TVHYLDLSLN PSGEVTKQIG DTLPVSCTIS ASRNATVVWM KDNIRLRSSP SFSSLHYQDA GNYVCETALQ EVEGLKKRES LTLIVEGKPQ IKMTKKTDPS GLSKTIICHV EGFPKPAIHW TITGSGSVIN QTEESPYING RYYSKIIISP EENVTLTCTA ENQLERTVNS LNVSAISIPE HDEADDISDE NREKVNDQAK Biological Activity: Measured by its ability to block the adhesion of Jurkat human acute T cell leukemia cells to immobilized Recombinant Human CD6. When cells are added to 1 ug/ml of mouse ALCAM binded to CD6, This effect is more to 50%. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 83.1
• NCBI: 033785
• UniProt: Q61490
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 50% glycerol.