TIE1, Recombinant, Human, His-Tag, aa22-759

Product Overview

• Article Name: TIE1, Recombinant, Human, His-Tag, aa22-759
• Biozol Catalog Number: USB-046008
• Supplier Catalog Number: 046008
• Alternative Catalog Number: USB-046008-20,USB-046008-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Tie-1, as known as tyrosine kinase with immunoglobulin like and EGF like domains 1, is an angiopoitein receptor. It is expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. However it has also been shown to be expressed in immature hematopoietic cells and platelets. It upregulates the cell adhesion molecules(CAMs) VCAM-1, E-selectin, and ICAM-1 through a p38-dependent mechanism. Also, It has a proinflammatory effect and may play a role in the endothelial inflammatory diseases such as atherosclerosis. Recombinant human Tie-1, fused to hIgG-His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa22-759 from human TIE1, fused to hlgG-His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~106.8kD (977aa) Amino Acid Sequence: AVDLTLLANL RLTDPQRFFL TCVSGEAGAG RGSDAWGPPL LLEKDDRIVR TPPGPPLRLA RNGSHQVTLR GFSKPSDLVG VFSCVGGAGA RRTRVIYVHN SPGAHLLPDK VTHTVNKGDT AVLSARVHKE KQTDVIWKSN GSYFYTLDWH EAQDGRFLLQ LPNVQPPSSG IYSATYLEAS PLGSAFFRLI VRGCGAGRWG PGCTKECPGC LHGGVCHDHD GECVCPPGFT GTRCEQACRE GRFGQSCQEQ CPGISGCRGL TFCLPDPYGC SCGSGWRGSQ CQEACAPGHF GADCRLQCQC QNGGTCDRFS GCVCPSGWHG VHCEKSDRIP QILNMASELE FNLETMPRIN CAAAGNPFPV RGSIELRKPD GTVLLSTKAI VEPEKTTAEF EVPRLVLADS GFWECRVSTS GGQDSRRFKV NVKVPPVPLA APRLLTKQSR QLVVSPLVSF SGDGPISTVR LHYRPQDSTM DWSTIVVDPS ENVTLMNLRP KTGYSVRVQL SRPGEGGEGA WGPPTLMTTD CPEPLLQPWL EGWHVEGTDR LRVSWSLPLV PGPLVGDGFL LRLWDGTRGQ ERRENVSSPQ ARTALLTGLT PGTHYQLDVQ LYHCTLLGPA SPPAHVLLPP SGPPAPRHLH AQALSDSEIQ LTWKHPEALP GPISKYVVEV QVAGGAGDPL WIDVDRPEET STIIRGLNAS TRYLFRMRAS IQGLGDWSNT VEESTLGNGL QAEGPVQESR AAEEGLDQ Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 106.8
• NCBI: 005415
• UniProt: P35590
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 50% glycerol.