NKp80, Recombinant, Human, His-Tag, aa60-231

Product Overview

• Article Name: NKp80, Recombinant, Human, His-Tag, aa60-231
• Biozol Catalog Number: USB-046009
• Supplier Catalog Number: 046009
• Alternative Catalog Number: USB-046009-20,USB-046009-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

NKp80/KLRF1, also known as killer cell lectin-like receptor subfamily F member 1, is a member of the C-type lectin family. Multiple splicing variants produce four isoforms for NKp80/KLRF1 and isoform 1 is the standard protein. It is expressed on NK cells and a subset of T lymphocytes whereas it is not expressed on B lymphocytes, monocytes and granulocytes. It stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies. It works through interaction with its ligand, AICL (activation-induced C-type lectin), which is selectively expressed on myeloid cells. NKp80-AICL interaction regulates the immune responses at sites of inflammation by stimulating the release of proinflammatory cytokines. Recombinant human NKp80/KLRF1, fused to hIgG-His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa60-231 from human NKp80, fused to hlgG-His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~47.1kD (414aa) Amino Acid Sequence: LLVSQGV LLKCQKGSCS NATQYEDTGD LKVNNGTRRN ISNKDLCASR SADQTVLCQS EWLKYQGKCY WFSNEMKSWS DSYVYCLERK SHLLIIHDQL EMAFIQKNLR QLNYVWIGLN FTSLKMTWTW VDGSPIDSKI FFIKGPAKEN SCAAIKESKI FSETCSSVFK WICQY Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to human KLRF1 coated plates 2ug/ml. This effect is more to 60%. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 47.1
• NCBI: 057607
• UniProt: Q9NZS2
• Purity: 85% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.