RNASE2, Recombinant, Human, His-Tag, aa28-161

Product Overview

• Article Name: RNASE2, Recombinant, Human, His-Tag, aa28-161
• Biozol Catalog Number: USB-046011
• Supplier Catalog Number: 046011
• Alternative Catalog Number: USB-046011-20,USB-046011-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

RNASE2, is a non-secretory ribonuclease that belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein is found in eosinophil granulocytes and closely related to the eosinophil cationic protein (RNASE3) from which it diverged 50 million years ago after the split between the old world and the new world monkeys. It is relatively neutral and has cytotoxic properties. It may generate host defense via roles in promoting leukocyte activation, maturation, and chemotaxis. It has also been studied in the context of defense against pathogen infection in mammals. Recombinant human RNASE2, fused to His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa28-161 from human RNASE2, fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~16.5kD (143aa) Amino Acid Sequence: KPPQFTW AQWFETQHIN MTSQQCTNAM QVINNYQRRC KNQNTFLLTT FANVVNVCGN PNMTCPSNKT RKNCHHSGSQ VPLIHCNLTT PSPQNISNCR YAQTPANMFY IVACDNRDQR RDPPQYPVVP VHLDRII Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 16.5
• NCBI: 002925
• UniProt: P10153
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.