IL-17A, Recombinant, Canine, His-Tag, aa29-155

Product Overview

• Article Name: IL-17A, Recombinant, Canine, His-Tag, aa29-155
• Biozol Catalog Number: USB-046014
• Supplier Catalog Number: 046014
• Alternative Catalog Number: USB-046014-20,USB-046014-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

IL-17A, also known as CTLA8, is a member of the IL-17 family of cytokines. This family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. The IL-17 family of cytokines includes six members, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F. IL-17A main funcion is stimulates production of IL-6, prostaglandin E and nitric oxide. And other inflammatory cytokines(TNF-alpha, IL-1 beta and IFN -gamma) leads to up-regulation of gene expression and progression and amplification of local inflammation. In addition, it serves induction of co-stimulatory molecules such as ICAM-1 and other cytokines, thereby supporting T cell activation. IL-17 expression has been associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. Recombinant canine IL-17A, fused to His -Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa29-155 from canine IL-17A, from His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~15.6kD (133aa) Amino Acid Sequence: FPQNPGCRNT EDKNFPQHVK VNLNILNRNT NSRRPSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CVNNEGNINY HMNSVPIQQE ILVLRRESQH CPHSFRLEKM LVAVGCTCVT PIVRHVA Biological Activity: The activity is determined by the IL-6 ELISA in a using NIH/3T3 mouse embryonic fibroblast cells. The ED50 range 10ng/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 15.6
• NCBI: 001159350
• UniProt: C6L8D7
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.