DKK1, Recombinant, Mouse, His-Tag, aa32-272

Product Overview

• Article Name: DKK1, Recombinant, Mouse, His-Tag, aa32-272
• Biozol Catalog Number: USB-046017
• Supplier Catalog Number: 046017
• Alternative Catalog Number: USB-046017-20,USB-046017-50,USB-046017-100,USB-046017-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Dkk-1, also known as Dickkopf-related protein 1, is a member of the Dkk protein family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Because of its antagonistic effects on the Wnt signaling pathway, it is believed that Dkk-1 is a common marker for neuronal death in neurodegenerative diseases like Alzheimers. Elevated level of Dkk-1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Recombinant mouse Dkk-1, fused to His-Tag at C-terminal, was expressed in HEK293 and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa32-272 from mouse DKK1, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~26.9kD (247aa) Amino Acid Sequence: TLNSVLINSN AIKNLPPPLG GAGGQPGSAV SVAPGVLYEG GNKYQTLDNY QPYPCAEDEE CGSDEYCSSP SRGAAGVGGV QICLACRKRR KRCMRHAMCC PGNYCKNGIC MPSDHSHFPR GEIEESIIEN LGNDHNAAAG DGYPRRTTLT SKIYHTKGQE GSVCLRSSDC AAGLCCARHF WSKICKPVLK EGQVCTKHKR KGSHGLEIFQ RCYCGEGLAC RIQKDHHQAS NSSRLHTCQR Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 26.9
• NCBI: 034181
• UniProt: O54908
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in 50mM MES, pH 6.5, 30% glycerol.