PKC iota, Recombinant, Human, His-Tag, aa1-596

Product Overview

• Article Name: PKC iota, Recombinant, Human, His-Tag, aa1-596
• Biozol Catalog Number: USB-046026
• Supplier Catalog Number: 046026
• Alternative Catalog Number: USB-046026-20,USB-046026-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

PKC iota, also known as Protein Kinase C iota, is a member of serine/threonine protein kinases family. It play a key regulatory role in a number of cellular functions including cell growth and differentiation, hormone secretion, and gene expression. It is involved in cell polarization processes and the formation of epithelial tight junctions. This protein kinase is calcium-independent and phospholipid-dependent. It is also implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. Recombinant human PKC iota, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-596 from human PKC iota, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~69kD (602aa) Amino Acid Sequence: MPTQRDSSTM SHTVAGGGSG DHSHQVRVKA YYRGDIMITH FEPSISFEGL CNEVRDMCSF DNEQLFTMKW IDEEGDPCTV SSQLELEEAF RLYELNKDSE LLIHVFPCVP ERPGMPCPGE DKSIYRRGAR RWRKLYCANG HTFQAKRFNR RAHCAICTDR IWGLGRQGYK CINCKLLVHK KCHKLVTIEC GRHSLPQEPV MPMDQSSMHS DHAQTVIPYN PSSHESLDQV GEEKEAMNTR ESGKASSSLG LQDFDLLRVI GRGSYAKVLL VRLKKTDRIY AMKVVKKELV NDDEDIDWVQ TEKHVFEQAS NHPFLVGLHS CFQTESRLFF VIEYVNGGDL MFHMQRQRKL PEEHARFYSA EISLALNYLH ERGIIYRDLK LDNVLLDSEG HIKLTDYGMC KEGLRPGDTT STFCGTPNYI APEILRGEDY GFSVDWWALG VLMFEMMAGR SPFDIVGSSD NPDQNTEDYL FQVILEKQIR IPRSLSVKAA SVLKSFLNKD PKERLGCHPQ TGFADIQGHP FFRNVDWDMM EQKQVVPPFK PNISGEFGLD NFDSQFTNEP VQLTPDDDDI VRKIDQSEFE GFEYINPLLM SAEECV Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 69
• NCBI: 002731
• UniProt: P41743
• Purity: 80% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.