TNFSF15, Recombinant, Human, His-Tag, aa72-251

Product Overview

• Article Name: TNFSF15, Recombinant, Human, His-Tag, aa72-251
• Biozol Catalog Number: USB-046029
• Supplier Catalog Number: 046029
• Alternative Catalog Number: USB-046029-20,USB-046029-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Tumor necrosis factor ligand superfamily member 15, also known TNFSF15, belongs to the TNF superfamily. TNFSF15 is predominantly expressed in endothelial cells and its expression is inducible by TNFalpha and IL1alpha. TNFSF15 is an angiogenesis inhibitor of the TNF family and functions in part by directly inhibiting endothelial cell proliferation. TNFSF15 may act as an autocrine factor to induce apoptosis in endothelial cells via activation of multiple signaling pathways, including stress protein kinases and certain caspases. Recombinant human TNFSF15 protein, fused to His-Tag at N-terminal, was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa72-251 from human TNFSF15, fused to His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~22.7kD (201aa) Amino Acid Sequence: LKGQEFAPS HQQVYAPLRA DGDKPRAHLT VVRQTPTQHF KNQFPALHWE HELGLAFTKN RMNYTNKFLL IPESGDYFIY SQVTFRGMTS ECSEIRQAGR PNKPDSITVV ITKVTDSYPE PTQLLMGTKS VCEVGSNWFQ PIYLGAMFSL QEGDKLMVNV SDISLVDYTK EDKTFFGAFL L Biological Activity: Measured by its ability to induce apoptosis in TF-1 human erythroleukemic cells in the presence of cycloheximaide. The ED50 range 100ng/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 22.7
• NCBI: 005109
• UniProt: O95150
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.5, 0.15 sodium chloride, 40% glycerol.