PRCP, Recombinant, Human, His-Tag, aa22-496

Product Overview

• Article Name: PRCP, Recombinant, Human, His-Tag, aa22-496
• Biozol Catalog Number: USB-046033
• Supplier Catalog Number: 046033
• Alternative Catalog Number: USB-046033-20,USB-046033-50,USB-046033-100,USB-046033-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

PRCP, also known as prolylcarboxypeptidase, is a member of the peptidase S28 family of serine exopeptidases. This protein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension 2, 3 and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. It has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension 2, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that PRCP may be related to essential hypertension. Recombinant human PRCP, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa22-496 from human PRCP, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~54.3kD (481aa) Amino Acid Sequence: LRPALRALGS LHLPTNPTSL PAVAKNYSVL YFQQKVDHFG FNTVKTFNQR YLVADKYWKK NGGSILFYTG NEGDIIWFCN NTGFMWDVAE ELKAMLVFAE HRYYGESLPF GDNSFKDSRH LNFLTSEQAL ADFAELIKHL KRTIPGAENQ PVIAIGGSYG GMLAAWFRMK YPHMVVGALA ASAPIWQFED LVPCGVFMKI VTTDFRKSGP HCSESIHRSW DAINRLSNTG SGLQWLTGAL HLCSPLTSQD IQHLKDWISE TWVNLAMVDY PYASNFLQPL PAWPIKVVCQ YLKNPNVSDS LLLQNIFQAL NVYYNYSGQV KCLNISETAT SSLGTLGWSY QACTEVVMPF CTNGVDDMFE PHSWNLKELS DDCFQQWGVR PRPSWITTMY GGKNISSHTN IVFSNGELDP WSGGGVTKDI TDTLVAVTIS EGAHHLDLRT KNALDPMSVL LARSLEVRHM KNWIRDFYDS AGKQ Biological Activity: Specific activity is > 3000pmol/min/µg, and is defined as the amount of enzyme that convert 1.0 pmole of Z-Pro-Ala-OH per minute at pH 4.0 at 25C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 54.3
• NCBI: 005031
• UniProt: P42785
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 30% glycerol.