IL-3R alpha, Recombinant, Mouse, His-Tag, aa17-331

Product Overview

• Article Name: IL-3R alpha, Recombinant, Mouse, His-Tag, aa17-331
• Biozol Catalog Number: USB-046036
• Supplier Catalog Number: 046036
• Alternative Catalog Number: USB-046036-20,USB-046036-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

IL-3R alpha/CD123, also known as interleukin 3 receptor subunit alpha, is a member of the type 1 cytokine receptor family and type 5 subfamily. It is expressed on multiple cell types, including endothelial cells, monocytes, eosinophils, basophils plus mast cells, and plasmacytoid CD4+ T cells. It serves as a low affinity binding protein for monomeric IL-3. The IL-3 and IL-3 R alpha complex likely interacts with a preformed signaling homodimer comprised of either beta IL-3 or beta c chains. The extracellular domain of mouse IL-3 R alpha/CD123 shares only 44% and 29% aa sequence identity with rat and human IL-3 R alpha, respectively. Nevertheless, human IL-3 is reported to be active on the mouse receptor complex that is expressed on select cell types. Also, the specific alpha subunit of the interleukin 3 receptor is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recombinant mouse IL-3R alpha/CD123, fused to hIgG-His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa17-331 from mouse IL-3R, fused to hlgG-His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~61.5kD (554aa) Amino Acid Sequence: SDLAAVREAP PTAVTTPIQN LHIDPAHYTL SWDPAPGADI TTGAFCRKGR DIFVWADPGL ARCSFQSLSL CHVTNFTVFL GKDRAVAGSI QFPPDDDGDH EAAAQDLRCW VHEGQLSCQW ERGPKATGDV HYRMFWRDVR LGPAHNRECP HYHSLDVNTA GPAPHGGHEG CTLDLDTVLG STPNSPDLVP QVTITVNGSG RAGPVPCMDN TVDLQRAEVL APPTLTVECN GSEAHARWVA RNRFHHGLLG YTLQVNQSSR SEPQEYNVSI PHFWVPNAGA ISFRVKSRSE VYPRKLSSWS EAWGLVCPPE VMPVK Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 61.5
• NCBI: 032395
• UniProt: P26952
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1 M sodium chloride, 30% glycerol.