Cathepsin C, Recombinant, Mouse, His-Tag, aa25-462

Product Overview

• Article Name: Cathepsin C, Recombinant, Mouse, His-Tag, aa25-462
• Biozol Catalog Number: USB-046038
• Supplier Catalog Number: 046038
• Alternative Catalog Number: USB-046038-20,USB-046038-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Cathepsin C/DPPI, also known as dipeptidyl peptidase I, is a lysosomal exo-cysteine protease belonging to the peptidase C1 protein family, a subgroup of the cysteine cathepsins. It catalyses excision of dipeptides from the N terminal of protein and peptide substrates, except if the amino group of the N-terminal is blocked. Also, It appears to be a central coordinator for activation of many serine proteases in immune/inflammatory cells. Defects in the Cathepsin C have been shown to be a cause of Papillon-Lefevre disease, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis. Also, It plays a key role in the activation of several degradative enzymes linked to tissue destruction in inflammatory diseases. Recombinant mouse Cathepsin C, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa25-462 from mouse Cathepsin C, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~50.5kD (444aa) Amino Acid Sequence: DTPANCTYPD LLGTWVFQVG PRSSRSDINC SVMEATEEKV VVHLKKLDTA YDELGNSGHF TLIYNQGFEI VLNDYKWFAF FKYEVRGHTA ISYCHETMTG WVHDVLGRNW ACFVGKKVES HIEKVNMNAA HLGGLQERYS ERLYTHNHNF VKAINTVQKS WTATAYKEYE KMSLRDLIRR SGHSQRIPRP KPAPMTDEIQ QQILNLPESW DWRNVQGVNY VSPVRNQESC GSCYSFASMG MLEARIRILT NNSQTPILSP QEVVSCSPYA QGCDGGFPYL IAGKYAQDFG VVEESCFPYT AKDSPCKPRE NCLRYYSSDY YYVGGFYGGC NEALMKLELV KHGPMAVAFE VHDDFLHYHS GIYHHTGLSD PFNPFELTNH AVLLVGYGRD PVTGIEYWII KNSWGSNWGE SGYFRIRRGT DECAIESIAV AAIPIPKL Biological Activity: Specific activity is > 50,000pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Gly-Arg-AMC per minute at pH 6.0 at 37C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 50.5
• NCBI: 034112
• UniProt: P97821
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.