IgG1 Fc, Recombinant, Human, aa100-330
Catalog Number:
USB-046039
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| Article Name: | IgG1 Fc, Recombinant, Human, aa100-330 |
| Biozol Catalog Number: | USB-046039 |
| Supplier Catalog Number: | 046039 |
| Alternative Catalog Number: | USB-046039-20,USB-046039-100,USB-046039-250,USB-046039-500 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| Immunoglobulins are membrane-bound or secreted glycoproteins produced by B lymphocytes. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors on phagocytic cells with high affinity. IgG comprise two identical light (L) and heavy (H) chains forming a 'Y-shaped structure. It also is digested by pepsin proteolysis into two distinct fragments (Fab, Fc). Fc fragment is a homodimeric glycoprotein with one monomer consisting of two constant immunoglobulin domains (CH2, CH3) from the C-terminal half of one heavy chain. Through interaction with its ligands (FcgammaR, C1q and FcRn) it mediates various immune effector functions and increases the half-life of the antibody molecule. Recombinant IgG Fc is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. Recombinant human IgG1 Fc was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa100-330 from human IgG1 Fc, expressed in HEK293. Molecular Weight: ~25.9kD (231aa) Amino Acid Sequence: PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K Biological Activity: 1. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a. The ED50 range 1.5ug/ml. 2. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a. 3. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a. The ED50 range 4ug/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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