FGF-8b, Recombinant, Human, His-Tag, aa23-215

Product Overview

• Article Name: FGF-8b, Recombinant, Human, His-Tag, aa23-215
• Biozol Catalog Number: USB-046042
• Supplier Catalog Number: 046042
• Alternative Catalog Number: USB-046042-20,USB-046042-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

FGF-8b, also known as Fibroblast growth factor 8 isoform B, is a member of the fibroblast growth factor (FGF) family that plays a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. There are 4 known alternate spliced forms of FGF 8, FGF-8a, FGF-8b, FGF-8e and FGF-8f. FGF-8 is widely expressed during embryogenesis, and mediates epithelial mesenchymal transitions. It encodes a key signaling factor, and its precise regulation is essential for embryo patterning. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity. FGF-8 signaling from the apical ectodermal ridge (AER), which borders the distal end of the limb bud, is necessary for forming normal limbs. FGF-8 is responsible for cell proliferation and survival, as well. The ability for FGF-8 to regulate cell proliferation has caused interest in its effects on tumors or squamous cell carcinoma. Recombinant human FGF 8b, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa23-215 from human FGF-8b, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~23.7kD (204aa) Amino Acid Sequence: QVTVQ SSPNFTQHVR EQSLVTDQLS RRLIRTYQLY SRTSGKHVQV LANKRINAMA EDGDPFAKLI VETDTFGSRV RVRGAETGLY ICMNKKGKLI AKSNGKGKDC VFTEIVLENN YTALQNAKYE GWYMAFTRKG RPRKGSKTRQ HQREVHFMKR LPRGHHTTEQ SLRFEFLNYP PFTRSLRGSQ RTWAPEPR Biological Activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells in the presence of 10ug/ml of heparin. The ED50 range 5ug/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 23.7
• NCBI: 006110
• UniProt: P55075
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.