CD16a, Recombinant, Human, His-Tag, aa18-208

Product Overview

• Article Name: CD16a, Recombinant, Human, His-Tag, aa18-208
• Biozol Catalog Number: USB-046043
• Supplier Catalog Number: 046043
• Alternative Catalog Number: USB-046043-20,USB-046043-50,USB-046043-100,USB-046043-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Fc gamma RIIIA/CD16a is also known as Low affinity immunoglobulin gamma Fc region receptor III-A isoform c. It is optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Also mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation. Involved in the generation of memory-like adaptive NK cells capable to produce high amounts of IFNG and to efficiently eliminate virus-infected cells via ADCC. Regulates NK cell survival and proliferation, in particular by preventing NK cell progenitor apoptosis. Fc gamma RIIIA is mediates the antitumor activities of therapeutic antibodies. Upon ligation on monocytes triggers TNFA-dependent ADCC of IgG-coated tumor cells. Recombinant human Fc gamma RIIIA/CD16a, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa18-208 from human CD16a, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~22.6kD (197aa) Amino Acid Sequence: MRTEDLPKAV VFLEPQWYRV LEKDSVTLKC QGAYSPEDNS TQWFHNESLI SSQASSYFID AATVDDSGEY RCQTNLSTLS DPVQLEVHIG WLLLQAPRWV FKEEDPIHLR CHSWKNTALH KVTYLQNGKG RKYFHHNSDF YIPKATLKDS GSYFCRGLFG SKNVSSETVN ITITQGLAVS TISSFFPPGY Q Biological Activity: Measured by its binding ability in a functional ELISA with Human IgG1 Fc. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 22.6
• NCBI: 001121065
• UniProt: P08637
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.