IL-4I1, Recombinant, Human, His-Tag, aa22-567

Product Overview

• Article Name: IL-4I1, Recombinant, Human, His-Tag, aa22-567
• Biozol Catalog Number: USB-046044
• Supplier Catalog Number: 046044
• Alternative Catalog Number: USB-046044-20,USB-046044-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

IL-4I1, also known as FIG1, is a secreted L-amino-acid oxidase that acts as a key immunoregulator. It is highly expressed in primary metastinal B-cell lymphomas. This protein acts as a negative regultaotr of anti-tumor immunity by mediating Trp degradation via ans indole pyruvate pathway that activates the transcription factor AHR. IL-4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer. IL-41I also regulates M2 macrophage polarization by inhibiting T-cell activation and has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4+ and H2O2. Recombinant human IL-4I1, fused to His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa22-567 from human IL-4I1, fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~61.8kD (555aa) Amino Acid Sequence: QDWKAER SQDPFEKCMQ DPDYEQLLKV VTWGLNRTLK PQRVIVVGAG VAGLVAAKVL SDAGHKVTIL EADNRIGGRI FTYRDQNTGW IGELGAMRMP SSHRILHKLC QGLGLNLTKF TQYDKNTWTE VHEVKLRNYV VEKVPEKLGY ALRPQEKGHS PEDIYQMALN QALKDLKALG CRKAMKKFER HTLLEYLLGE GNLSRPAVQL LGDVMSEDGF FYLSFAEALR AHSCLSDRLQ YSRIVGGWDL LPRALLSSLS GLVLLNAPVV AMTQGPHDVH VQIETSPPAR NLKVLKADVV LLTASGPAVK RITFSPPLPR HMQEALRRLH YVPATKVFLS FRRPFWREEH IEGGHSNTDR PSRMIFYPPP REGALLLASY TWSDAAAAFA GLSREEALRL ALDDVAALHG PVVRQLWDGT GVVKRWAEDQ HSQGGFVVQP PALWQTEKDD WTVPYGRIYF AGEHTAYPHG WVETAVKSAL RAAIKINSRK GPASDTASPE GHASDMEGQG HVHGVASSPS HDLAKEEGSH PPVQGQLSLQ NTTHTRTSH Biological Activity: Specific activity is > 300pmol/min/ug, and defined as the amount of enzyme that oxidize 3-phenylpyruvate at pH 7.0 at 25C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 61.8
• NCBI: 690863
• UniProt: Q96RQ9
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in 20mM MES, pH 5.5, 0.1M sodium chloride, 40% glycerol.