B7-H6, Recombinant, Human, His-Tag, aa25-262

Product Overview

• Article Name: B7-H6, Recombinant, Human, His-Tag, aa25-262
• Biozol Catalog Number: USB-046045
• Supplier Catalog Number: 046045
• Alternative Catalog Number: USB-046045-20,USB-046045-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

B7-H6, also known as NCR3LG1, is a member of the B7 family of immune co-stimulatory proteins. B7-H6 is as a cell-surface ligand for the NKp30-activating receptor expressed on natural killer cells. It is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, and is consistent with the detection of NKp30 binding sites on many tumors. The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30-dependent cell lysis. Recombinant human B7-H6, fused to hIgG-His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa25-262 from human B7-H6, hlgG-His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~53.6kD (477aa) Amino Acid Sequence: DLKVEMMAGG TQITPLNDNV TIFCNIFYSQ PLNITSMGIT WFWKSLTFDK EVKVFEFFGD HQEAFRPGAI VSPWRLKSGD ASLRLPGIQL EEAGEYRCEV VVTPLKAQGT VQLEVVASPA SRLLLDQVGM KENEDKYMCE SSGFYPEAIN ITWEKQTQKF PHPIEISEDV ITGPTIKNMD GTFNVTSCLK LNSSQEDPGT VYQCVVRHAS LHTPLRSNFT LTAARHSLSE TEKTDNFS Biological Activity: Measured by its binding ability in a functional ELISA with Human NCR3. The ED50 range 500ng/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 53.6
• NCBI: 001189368
• UniProt: Q68D85
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.