POR, Recombinant, Human, His-Tag, aa43-677

Product Overview

• Article Name: POR, Recombinant, Human, His-Tag, aa43-677
• Biozol Catalog Number: USB-046052
• Supplier Catalog Number: 046052
• Alternative Catalog Number: USB-046052-20,USB-046052-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

POR, also known as NADPH--cytochrome P450 reductase, is a flavoprotein that donates electrons to all microsomal P450 enzymes. POR is localized to the endoplasmic reticulum, where it is also able to transfer electrons to heme oxygenase and cytochrome b5. It is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase and flavodoxin. Recombinant human POR, fused to His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa43-677 from human POR, from His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~73kD (642aa) Amino Acid Sequence: MLFRKKKEEV PEFTKIQTLT SSVRESSFVE KMKKTGRNII VFYGSQTGTA EEFANRLSKD AHRYGMRGMS ADPEEYDLAD LSSLPEIDNA LVVFCMATYG EGDPTDNAQD FYDWLQETDV DLSGVKFAVF GLGNKTYEHF NAMGKYVDKR LEQLGAQRIF ELGLGDDDGN LEEDFITWRE QFWPAVCEHF GVEATGEESS IRQYELVVHT DIDAAKVYMG EMGRLKSYEN QKPPFDAKNP FLAAVTTNRK LNQGTERHLM HLELDISDSK IRYESGDHVA VYPANDSALV NQLGKILGAD LDVVMSLNNL DEESNKKHPF PCPTSYRTAL TYYLDITNPP RTNVLYELAQ YASEPSEQEL LRKMASSSGE GKELYLSWVV EARRHILAIL QDCPSLRPPI DHLCELLPRL QARYYSIASS SKVHPNSVHI CAVVVEYETK AGRINKGVAT NWLRAKEPAG ENGGRALVPM FVRKSQFRLP FKATTPVIMV GPGTGVAPFI GFIQERAWLR QQGKEVGETL LYYGCRRSDE DYLYREELAQ FHRDGALTQL NVAFSREQSH KVYVQHLLKQ DREHLWKLIE GGAHIYVCGD ARNMARDVQN TFYDIVAELG AMEHAQAVDY IKKLMTKGRY SLDVWS Biological Activity: Specific activity is > 1500pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole cytochrome C by NADPH per minute at pH8.0 at 25C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 73
• NCBI: 001382342
• UniProt: P16435
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.