CD4, Recombinant, Human, His-Tag, aa26-390

Product Overview

• Article Name: CD4, Recombinant, Human, His-Tag, aa26-390
• Biozol Catalog Number: USB-046053
• Supplier Catalog Number: 046053
• Alternative Catalog Number: USB-046053-20,USB-046053-50,USB-046053-100,USB-046053-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

CD4, also known as T-cell surface antigen T4/Leu-3, is a member of the immunoglobulin superfamily. It is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. It is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Also, it binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. Recombinant human CD4, fused to hIgG-His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa26-390 from human CD4, fused to hlgG-His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~67.7kD (604aa) Amino Acid Sequence: KKVVLGKKGD TVELTCTASQ KKSIQFHWKN SNQIKILGNQ GSFLTKGPSK LNDRADSRRS LWDQGNFPLI IKNLKIEDSD TYICEVEDQK EEVQLLVFGL TANSDTHLLQ GQSLTLTLES PPGSSPSVQC RSPRGKNIQG GKTLSVSQLE LQDSGTWTCT VLQNQKKVEF KIDIVVLAFQ KASSIVYKKE GEQVEFSFPL AFTVEKLTGS GELWWQAERA SSSKSWITFD LKNKEVSVKR VTQDPKLQMG KKLPLHLTLP QALPQYAGSG NLTLALEAKT GKLHQEVNLV VMRATQLQKN LTCEVWGPTS PKLMLSLKLE NKEAKVSKRE KAVWVLNPEA GMWQCLLSDS GQVLLESNIK VLPTW Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. When cells are added to human CD4 coated plates. The ED50 range 10ug/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 67.7
• NCBI: 000607
• UniProt: P01730
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 40% glycerol.