BACE-2, Recombinant, Mouse, His-Tag, aa20-462

Product Overview

• Article Name: BACE-2, Recombinant, Mouse, His-Tag, aa20-462
• Biozol Catalog Number: USB-046055
• Supplier Catalog Number: 046055
• Alternative Catalog Number: USB-046055-20,USB-046055-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

BACE-2, also known as beta secretase 2, belongs to the peptidase A1 family. It is an aspartic protease that is a close homolog of BACE-1. However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in Abeta peptide production. It has been shown to play a key role in insulin receptor trafficking in the pancreas where it is expressed in beta cells and affects glucose tolerance and was suggested as a promising target for improving beta cell function in diabetes. Recombinant mouse BACE-2, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa20-462 from mouse BACE-2, His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~48.6kD (449aa) Amino Acid Sequence: AVPALAPAPF TLPLQVARAT NHRASAVPGL GTPELPRADG LALALEPVRA TANFLAMVDN LQGDSGRGYY LEMLIGTPPQ KVQILVDTGS SNFAVAGAPH SYIDTYFDSE SSSTYHSKGF DVTVKYTQGS WTGFVGEDLV TIPKGFNSSF LVNIATIFES ENFFLPGIKW NGILGLAYAA LAKPSSSLET FFDSLVAQAK IPDIFSMQMC GAGLPVAGSG TNGGSLVLGG IEPSLYKGDI WYTPIKEEWY YQIEILKLEI GGQNLNLDCR EYNADKAIVD SGTTLLRLPQ KVFDAVVEAV ARTSLIPEFS DGFWTGAQLA CWTNSETPWA YFPKISIYLR DENASRSFRI TILPQLYIQP MMGAGFNYEC YRFGISSSTN ALVIGATVME GFYVVFDRAQ RRVGFAVSPC AEIEGTTVSE ISGPFSTEDI ASNCVPAQAL NEP Biological Activity: Specific activity is > 20pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK (Dnp) RR-NH2 to Mca- Pro-Leu-OH per minute at pH 3.5 at 25C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 48.6
• NCBI: 062390
• UniProt: Q9JL18
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.