FGF basic, Recombinant, Human, aa143-288
Catalog Number:
USB-046058
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| Article Name: | FGF basic, Recombinant, Human, aa143-288 |
| Biozol Catalog Number: | USB-046058 |
| Supplier Catalog Number: | 046058 |
| Alternative Catalog Number: | USB-046058-20,USB-046058-100 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| Fibroblast growth factor 2 (FGF2) is a member of the FGF family that binds heparin and plays important roles in morphogenic, neurotrophic and angiogenic processes. FGF2 possess diverse biological functions, such as neuron differentiation, embryonic development and differentiation, modulation of angiogenesis, and wound healing. Recombinant human FGF2 was expressed in E. coli and purified by conventional chromatography techniques. Source: Recombinant protein corresponding to aa143-288 from human FGF basic, expressed in E.coli. Molecular Weight: ~16.5kD (147aa) Amino Acid Sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS Biological Activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells in the presence of 10ug/ml of heparin. The ED50 range 0.2ng/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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