Lactate Dehydrogenase A, Recombinant, Mouse, His-Tag, aa1-332

Product Overview

• Article Name: Lactate Dehydrogenase A, Recombinant, Mouse, His-Tag, aa1-332
• Biozol Catalog Number: USB-046059
• Supplier Catalog Number: 046059
• Alternative Catalog Number: USB-046059-20,USB-046059-50,USB-046059-100,USB-046059-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Ldha, also known as L-lactate dehydrogenase A chain isoform 1, is a member of the LDH/MDH superfamily and LDH family. It catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. This protein is found predominantly in muscle tissue. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It plays an important role in the development, invasion and metastasis of malignancies. Recombinant mouse Ldha, fused to His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-332 from mouse Lactate Dehydrogenase A, fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~37.5kD (340aa) Amino Acid Sequence: MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QF Biological Activity: Specific activity is > 250unit/mg, and is defined as the Amount of enzyme that convert 1.0umole of pyruvate to L-lactate and per minute at pH 7.5 at 37°C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 37.5
• NCBI: 034829
• UniProt: P06151
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.