ICAM-1, Recombinant, Mouse, His-Tag, aa28-485

Product Overview

• Article Name: ICAM-1, Recombinant, Mouse, His-Tag, aa28-485
• Biozol Catalog Number: USB-046060
• Supplier Catalog Number: 046060
• Alternative Catalog Number: USB-046060-20,USB-046060-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

ICAM-1, also known as CD54 (Cluster of Differentiation 54), belongs to the ICAM proteins. This protein is ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. During leukocyte trans-endothelial migration, ICAM-1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. It is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. Also, It has been implicated in the progression of autoimmune diseases. Recombinant mouse ICAM1, fused to His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa28-485 from mouse ICAM-1, fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~51.2kD (466aa) Amino Acid Sequence: QVSIHPREAF LPQGGSVQVN CSSSCKEDLS LGLETQWLKD ELESGPNWKL FELSEIGEDS SPLCFENCGT VQSSASATIT VYSFPESVEL RPLPAWQQVG KDLTLRCHVD GGAPRTQLSA VLLRGEEILS RQPVGGHPKD PKEITFTVLA SRGDHGANFS CRTELDLRPQ GLALFSNVSE ARSLRTFDLP ATIPKLDTPD LLEVGTQQKL FCSLEGLFPA SEARIYLELG GQMPTQESTN SSDSVSATAL VEVTEEFDRT LPLRCVLELA DQILETQRTL TVYNFSAPVL TLSQLEVSEG SQVTVKCEAH SGSKVVLLSG VEPRPPTPQV QFTLNASSED HKRSFFCSAA LEVAGKFLFK NQTLELHVLY GPRLDETDCL GNWTWQEGSQ QTLKCQAWGN PSPKMTCRRK ADGALLPIGV VKSVKQEMNG TYVCHAFSSH GNVTRNVYLT VLYHSQNN Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM-1/CD54 coated plates 5ug/ml. This effect is more to 60%. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 51.2
• NCBI: 034623
• UniProt: P13597
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.