APP/Protease Nexin II, Recombinant, Human, His-Tag, aa18-701

Article Name:	APP/Protease Nexin II, Recombinant, Human, His-Tag, aa18-701
Biozol Catalog Number:	USB-046063
Supplier Catalog Number:	046063
Alternative Catalog Number:	USB-046063-20,USB-046063-100
Manufacturer:	US Biological
Category:	Molekularbiologie

APP, also known as amyloid beta A4 protein, functions as a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. It has been implicated as a regulator of synapse formation, neural plasticity, antimicrobial activity, and iron export. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebral arterial amyloidosis. Recombinant human APP, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa18-701 from human APP/Protease Nexin II, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~78.2kD (690aa) Amino Acid Sequence: LEVPTDGNAG LLAEPQIAMF CGRLNMHMNV QNGKWDSDPS GTKTCIDTKE GILQYCQEVY PELQITNVVE ANQPVTIQNW CKRGRKQCKT HPHFVIPYRC LVGEFVSDAL LVPDKCKFLH QERMDVCETH LHWHTVAKET CSEKSTNLHD YGMLLPCGID KFRGVEFVCC PLAEESDNVD SADAEEDDSD VWWGGADTDY ADGSEDKVVE VAEEEEVAEV EEEEADDDED DEDGDEVEEE AEEPYEEATE RTTSIATTTT TTTESVEEVV REVCSEQAET GPCRAMISRW YFDVTEGKCA PFFYGGCGGN RNNFDTEEYC MAVCGSAMSQ SLLKTTQEPL ARDPVKLPTT AASTPDAVDK YLETPGDENE HAHFQKAKER LEAKHRERMS QVMREWEEAE RQAKNLPKAD KKAVIQHFQE KVESLEQEAA NERQQLVETH MARVEAMLND RRRLALENYI TALQAVPPRP RHVFNMLKKY VRAEQKDRQH TLKHFEHVRM VDPKKAAQIR SQVMTHLRVI YERMNQSLSL LYNVPAVAEE IQDEVDELLQ KEQNYSDDVL ANMISEPRIS YGNDALMPSL TETKTTVELL PVNGEFSLDD LQPWHSFGAD SVPANTENEV EPVDARPAAD RGLTTRPGSG LTNIKTEEIS EVKMDAEFRH DSGYEVHHQK LVFFAEDVGS NKGA Biological Activity: The IC50 value is 1nM, The inhibitory function of APP on activity of trypsin was measured by a fluorometric assay using Mca-RPKPVE-Nval-WRK(Dnp)-NH2 at pH 7.5 at 37C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:	78.2
NCBI:	000475
UniProt:	P05067
Purity:	90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
Form:	Supplied as a liquid in PBS, pH 7.4, 10% glycerol.