DSPG3, Recombinant, Human, His-Tag, aa20-322

Product Overview

• Article Name: DSPG3, Recombinant, Human, His-Tag, aa20-322
• Biozol Catalog Number: USB-046064
• Supplier Catalog Number: 046064
• Alternative Catalog Number: USB-046064-20,USB-046064-50,USB-046064-100,USB-046064-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

DSPG3, also called epiphycan/EPYC or PG-Lb, is a class III subfamily member of the SLRP(small leucine-rich proteoglycans) family. DSPG3 is found predominantly in epiphyseal cartilage, although DSPG3 mRNA is also detected in mouse testis and human ligament and placenta. In mouse, expression begins in mid-gestation as an intermediate marker for chondrogenesis, peaks at birth and declines thereafter. Deletion of DSPG3 in mouse results in a mild postnatal phenotype that worsens synergistically when the co-expressed SLRP biglycan is also deleted. The double-mutant phenotype includes premature osteoarthritis, indicating a role for DSPG3, in cooperation with other SLRPs, in stability of the collagen matrix and maintenance of joint integrity. Recombinant human DSPG3, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa20-322 from human DSPG3, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~35.5kD (309aa) Amino Acid Sequence: APTLESINYD SETYDATLED LDNLYNYENI PVDKVEIEIA TVMPSGNREL LTPPPQPEKA QEEEEEEEST PRLIDGSSPQ EPEFTGVLGP HTNEDFPTCL LCTCISTTVY CDDHELDAIP PLPKNTAYFY SRFNRIKKIN KNDFASLSDL KRIDLTSNLI SEIDEDAFRK LPQLRELVLR DNKIRQLPEL PTTLTFIDIS NNRLGRKGIK QEAFKDMYDL HHLYLTDNNL DHIPLPLPEN LRALHLQNNN ILEMHEDTFC NVKNLTYIRK ALEDIRLDGN PINLSKTPQA YMCLPRLPVG SLV Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of Saos-2 human osteosarcoma cells. When cells are added to human DSPG3 1.25ug/ml and human fibronectin 0.5ug/ml coated plates. This effect is more to 60%. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 35.5
• NCBI: 004941
• UniProt: Q99645
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.