Fc epsilon RI alpha, Recombinant, Human, His-Tag, aa26-205

Product Overview

• Article Name: Fc epsilon RI alpha, Recombinant, Human, His-Tag, aa26-205
• Biozol Catalog Number: USB-046065
• Supplier Catalog Number: 046065
• Alternative Catalog Number: USB-046065-20,USB-046065-50,USB-046065-100,USB-046065-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Fc epsilon RI alpha, also known as FCER1A, is an IgE-binding protein of the multichain immune recognition family. Fc epsilon receptor is a tetrameric complex of one alpha, one beta and two gamma subunits on mast cells and basophils. This protein represents the alpha subunit, only the alpha subunit is glycosylated. The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response. Recombinant human Fc epsilon RI alpha/FCER1A, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa26-205 from human Fc epsilon RI alpha, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~21.8kD (186aa) Amino Acid Sequence: VPQKPKVSLN PPWNRIFKGE NVTLTCNGNN FFEVSSTKWF HNGSLSEETN SSLNIVNAKF EDSGEYKCQH QQVNESEPVY LEVFSDWLLL QASAEVVMEG QPLFLRCHGW RNWDVYKVIY YKDGEALKYW YENHNISITN ATVEDSGTYY CTGKVWQLDY ESEPLNITVI KAPREKYWLQ Biological Activity: Measured by its binding ability in a functional ELISA with Human IgE. The ED50 range 0.01ug/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 21.8
• NCBI: 001374209
• UniProt: P12319
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.