Source: Recombinant protein corresponding to Met1~Thr212 from human Mediator Complex Subunit 1, fused to HisTag at N-terminal, expressed in E. coli. Molecular Weight: ~27.5kD Applications: Suitable for use as a positive control, immunogen, SDS-PAGE and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute in 20mM Tris, 150mM sodium chloride (pH 8.0) to a concentration of 0.1-1.0mg/ml. Do not vortex. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder in 20mM Tris, 150mM sodium chloride, pH 8.0, containing 1mM EDTA, 1mM DTT, 0.01% skl, 5% Trehalose and Proclin300. Reconstitute in 20mM Tris, 150mM sodium chloride (pH 8.0) to a concentration of 0.1-1.0mg/ml. Do not vortex
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