Recombinant protein corresponding to Cys839~Gln1015 from human Tyrosine Phosphatase Receptor Type N2, with a N-terminal His-Tag, expressed in E. coli. Molecular Weight: ~24.0kD Applications: Suitable for use as a positive control, immunogen, SDS-PAGE and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as a lyophilized powder in 100mMNaHCO3, 500mM sodium chloride, pH 8.3, containing 1mM EDTA,1mM DTT, 0.01% SKL, 5% Trehalose. Reconstitute with ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex
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