Eukaryotic Calreticulin (CALR), Recombinant, Human, His-Tag

Catalog Number: USB-050333
Article Name: Eukaryotic Calreticulin (CALR), Recombinant, Human, His-Tag
Biozol Catalog Number: USB-050333
Supplier Catalog Number: 050333
Alternative Catalog Number: USB-050333-10,USB-050333-50,USB-050333-100,USB-050333-200
Manufacturer: US Biological
Category: Molekularbiologie
Application: WB
Recombinant protein corresponding to Glu18~Leu417 from human Eukaryotic Calreticulin, with a N-terminal His-Tag, expressed in 293F cells. Molecular Weight: ~48.1kD Applications: Suitable for use as a positive control, immunogen, SDS-PAGE and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight: 48.1
UniProt: P27797
Purity: 95%.
Form: Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with sterile 10mM PBS, pH 7.4 to 0.1-1mg/ml. Do not vortex