Proteinase 3 (PR3), Recombinant, Human, His-Tag

Catalog Number: USB-051470
Article Name: Proteinase 3 (PR3), Recombinant, Human, His-Tag
Biozol Catalog Number: USB-051470
Supplier Catalog Number: 051470
Alternative Catalog Number: USB-051470-10,USB-051470-50,USB-051470-100,USB-051470-200
Manufacturer: US Biological
Category: Molekularbiologie
Application: WB
Recombinant protein corresponding to Ile28~Arg248 from human Proteinase 3, with a N-terminal His-Tag, expressed in E. coli. Molecular Weight: ~27.9kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight: 27.9
UniProt: P24158
Purity: 90%.
Form: Supplied as a lyophilized powder in PBS, pH 7.4, 0.01% SKL, 5% trehalose. Reconstitute with sterile 10mM PBS, pH 7.4 to 0.1-1mg/ml. Do not vortex.