Recombinant protein corresponding to Ala25~Ala237 from human Cholinergic Receptor, Nicotinic, Alpha 10, with a N-terminal His-Tag, expressed in E. coli. Molecular Weight: ~27.6kD Applications: Suitable for use as a positive control, immunogen, SDS-PAGE and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder in 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with ddH2O to a concentration of 0.1-0.3mg/ml. Do not vortex.
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