Bone Morphogenetic Protein 4, Recombinant, Human (BMP4, BMP-2B) (Carrier Free)
Biozol Catalog Number:
USB-051944
Supplier Catalog Number:
051944
Alternative Catalog Number:
USB-051944-10
Manufacturer:
US Biological
Category:
Molekularbiologie
BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein. Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfide-linked heterodimer. Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation. In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm. BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans. Recombinant protein corresponding to Ser293-Arg408 from human BMP-4, expressed in mouse myeloma cell line, NSO. Uniprot/Swiss Accession: Q53XC5 N-Terminal Sequence Analysis: Ser293 Predicted Molecular Mass: 13kD (monomer) SDS-PAGE: 19-24kD (reducing conditions). 35-41kD (non-reducing conditions). Biological Activity: 1. Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.7-7ng/mL. 2. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 3-30ng/mL. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile 4mM HCl. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
