Chlamydia trachomatis

Product Overview

• Article Name: Chlamydia trachomatis
• Biozol Catalog Number: USB-051968
• Supplier Catalog Number: 051968
• Alternative Catalog Number: USB-051968-1
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: ELISA

Product Description

Chlamydia trachomatis is a nonmotile, Gram-negative human pathogen. It undergoes a unique biphasic developmental cycle, forming elementary bodies extracellularly and distinctive intracellular inclusions. The elementary body is infectious but metabolically inactive, cannot replicate and lacks detectable peptidoglycan. The major outer membrane protein (MOMP) comprises a large percentage of the outer membrane and is a transmembrane protein with type, species and genus reactive epitopes. The outer membrane also carries a heat stable lipopolysaccharide containing the reactive epitopes used for genus specific serological tests. There is structural similarity and antigenic cross-reactivity between chlamydial and other Gram-negative LPS moieties. Chlamydia trachomatis is a common sexually transmitted bacterium. Chlamydia infection may cause proctitis, urethritis, trachoma (an eye infection and the leading cause of blindness worldwide) and infertility in both sexes. Prostatitis and epididymitis may occur in men and in women infection might lead to acute or chronic pelvic pain, pelvic inflammatory disease, cervicitis and ectopic pregnancy. In neonates the pathogen may cause trachoma and pulmonary complications. 051968 contains a high concentration of elementary bodies and chlamydial components in phosphate buffered saline. Residual host cellular material is present. Mouse L cells are infected with C. trachomatis elementary bodies. Optimally infected cells are harvested and disrupted by sonication in phosphate bufffered saline. Cellular debris is removed by centrifugation. Source: Chlamydia trachomatis, LGV2 strain 434, cultured in mouse L cells Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: Gamma radiation, which primarily damages chlamydial genetic material.. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: No test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.