Erythropoietin (EPO), Recombinant, Human (Carrier Free)
Biozol Catalog Number:
USB-051975
Supplier Catalog Number:
051975
Alternative Catalog Number:
USB-051975-10,USB-051975-25
Manufacturer:
US Biological
Category:
Molekularbiologie
Human Erythropoietin (EPO) was initially purified from urine of a patient with aplastic anemia. EPO is a glycoprotein composed of 40-60% carbohydrates, and its molecular weight varies from 30-34kD depending on the carbohydrate percentage. Hypoxia induces erythropoiesis, and hypoxia inducible factor (HIF) is directly involved in EPO expression. HIF is a heterodimer (HIF-1alpha, HIF-2alpha, and beta subunits) transcription factor, and it is regulated by HIF-prolyl hydroxylase (HIF-PH) that hydroxylates the alpha subunits, targeting them for ubiquitination and subsequent degradation. HIF-PH is a sensor of levels of iron, oxygen, and metabolic activity. High levels of HIF protein induce EPO production in the kidney and liver, and mobilization of iron to support erythropoiesis. EPO binds to the EPOR, and transcripts of this receptor have been detected in non-erythroid precursors such as endothelial cells and renal proximal epithelial cells, nevertheless, it has been published that functional erythropoietin receptor is not detected in endothelial, cardiac, neuronal, and renal cells. Several transcriptions factors play a role in the expression of EPOR such as GATA 1, Friend of GATA (Fog1), and the erythroid specific factor SCL/Tal 1. EPO has been used in the treatment of anemia associated with chronic kidney disease, in cancer patients on chemotherapy, and in antiviral HIV therapy. Recombinant protein corresponding to Ala28-Arg192 from human Erythropoietin, expressed in CHO cells. Molecular Weight: ~18kD. The DTT-reduced and non-reduced protein migrate at ~37-57kD and 35-55kD by SDS-PAGE respectively. The N-terminal amino acid is Ala. Biological Activity: As determined by the dose dependent stimulation of TF-1 cells proliferation the ED50 is 0.1-0.6ng/ml, corresponding to a specific activity of 1.67-10x10e6 units/mg. Applications: Suitable for use in Bioassays. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
