The mCherry protein is derived from DsRed, a red fluorescent protein from the disc coral genus Discosoma. The protein is similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties. Several further cycles of mutation, directed modification and evolutionary selection produced mCherry. The mCherry protein sequence was deposited in NCBI entry AY678264, identical to that in Uniprot entry X5DSL3, and is also identical to that found in many expression vectors such as pGGD003. Recombinant construct expressed in and purified from E. coli using the pET29a (+) vector. This vector adds a few N and C-terminal amino acids. The N terminus contains an S-tag, which can be used to purify the protein. A C-terminal His-tag was used to purify the protein. The sequence is identical to that found in a series of widely used expression vectors Full length recombinant protein corresponding to mCherry, fused to His-Tag at C-terminal, expressed in E. coli. Swiss/Uniprot: X5DSL3 Molecular Weight: ~34kD Amino Acid Sequence: MKETAAAKFE RQHMDSPDLG TLVPRGSMAD IGSEFMVSKG EEDNMAIIKE FMRFKVHMEG SVNGHEFEIE GEGEGRPYEG TQTAKLKVTK GGPLPFAWDI LSPQFMYGSK AYVKHPADIP DYLKLSFPEG FKWERVMNFE DGGVVTVTQD SSLQDGEFIY KVKLRGTNFP SDGPVMQKKT MGWEASSERM YPEDGALKGE IKQRLKLKDG GHYDAEVKTT YKAKKPVQLP GAYNVNIKLD ITSHNEDYTI VEQYERAEGR HSTGGMDELY KVDKLAAALE HHHHHH Applications: Suitable for use as a fluorescent protein standard and as an immunogen for antibody production. Other applications not tested. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
