Protein Disulfide Isomerase/P4HB, Recombinant, Human (Proline 4-Hydroxylase, Beta Polypeptide)
Biozol Catalog Number:
USB-052052
Supplier Catalog Number:
052052
Alternative Catalog Number:
USB-052052-50
Manufacturer:
US Biological
Category:
Molekularbiologie
Protein Disulfide Isomerase, also known as prolyl 4-hydroxylase subunit beta (P4HB), protocollagen hydroxylase, cellular thyroid hormone binding protein p55 and glutathione-insulin transhydrogenase (1-3) is an abundant multifunctional enzyme that belongs to the Protein Disulfide Isomerase family. It contains two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. When present as a tetramer consisting of two alpha subunits and two beta subunits, this enzyme functions as a hydroxylase catalyzing the hydroxylation of prolyl residues in preprocollagen. P4HB has various additional functions (4-7). It binds thyroid hormone. It acts as a chaperone that inhibits aggregation of misfolded proteins. It plays a role in both the influx and efflux of S-nitrosothiol-bound nitric oxide. It is also a subunit of the microsomal triglyceride transfer protein complex. Recombinant protein corresponding to Asp18-Lys505 from human Protein Disulfide Isomerase/P4HB, fused to 10-His tag at C-terminal, expressed in CHO cells. Swiss/Uniprot Accession: P07237 Molecular Weight: ~60kD, reducing conditions N-terminal Sequence Analysis: Asp18 Biological Activity: Measured by its ability to promote aggregation of insulin in the presence of DTT Specific Activity: >7.5 A650/cm/min/mg Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
