TGF-beta family members are key modulators of cell proliferation, differentiation, matrix synthesis, and apoptosis. As implied by their name, BMPs initiate, promote, and regulate the development, growth, and remodeling of bone and cartilage. In addition to this role, BMPs are also involved in prenatal development and postnatal growth, remodeling, and maintenance of a variety of other tissues and organs. BMP-7, also known as osteogenic protein-1 or OP-1, is a potent bone inducing agent, which in the presence of an appropriate osteoconductive carrier (e.g. collagen sponge or synthetic hydroxyapatite) can be used in the treatment of bone defects. A bone-graft substitute, called OP-1TM implant, made of recombinant human BMP-7 associated with bovine bone-derived collagen, has recently been approved by the FDA as a device for treating critical-size bone fractures. The potential use of BMP-7 in dental reconstructive surgeries is currently under investigation. Recombinant human BMP-7 is a homodimeric glycoprotein consisting of two 117 amino acid subunits, which correspond to amino acid residues 315-431 of the full-length BMP-7 precursor. The calculated molecular weight is 26.4kD, however, due to glycosylation, the BMP-7 homodimer migrates at an apparent molecular weight of approximately 25-35kD by SDS-PAGE analysis under non-reducing conditions. Molecular Weight: ~26.4kD Amino Acid Sequence: MANVAENSSS DQRQACKKHE LYVSFRDLGW QDWIIAPEGY AAYYCEGECA FPLNSYMNAT NHAIVQTLVH FINPETVPKP CCAPTQLNAI SVLYFDDSSN VILKKYRNMV VRACGCH Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.02-0.04ug/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to 0.1-1mg/ml. This solution can then be diluted into other aqueous solutions with 0.1% HSA or BSA.
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